University of Guilan Aquatic Animals Nutrition 2980-8499 3 1 2017 03 21 Evaluation of antimicrobial properties of diethyl ether and methanol extracts of Scenedesmus dimorphus against bacterial Micrococcus lutesus and Aeromonas hydrophila Evaluation of antimicrobial properties of diethyl ether and methanol extracts of Scenedesmus dimorphus against bacterial Micrococcus lutesus and Aeromonas hydrophila 35 45 3154 10.22124/janb.2017.3154 FA Zahra Habibi Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran Javid Imanpour Namin Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran Zohreh Remazanpour International Sturgeon Research Organization of the Caspian Sea, Agricultural Research, Education and Extension Organization, Rasht, Guilan, Iran Journal Article 2016 10 22 Antimicrobial properties of diethyl ether and methanol extracts of algae Scenedesmus dimorphus against Gram positive bacterium Micrococcus lutesus and Gram negative becterium, Aeromonas hydrophila were evaluated. The algae was pure cultured and once the required volume and number of cells achieved coli using agar well diffusion method diethylether and hexane extracts of the algae cells were extracted. Antibacterial properties of the extracts were evaluated using well diffusion method. The results showed inhibitory activities of methanol and ether extracts of Scenedesmus dimorphus against M. luteus and A. hydrophila with 11-13 mm and 13-21 mm diameters of inhibition zone, respectively. In case of diethyl ether extracts also the inhibitory activity observed with diamaters of inhibition zone in the range of 9-23.7 mm and 21.6-6.3 mm respectively against M. luteus and A. hydrophila. Gas chromatography mass spectrophotometry was used to identify different substances within diethyl ether and methanol extracts with hydrocarbon and ester with highest perentage among other substances. The results confirmed antimicrobial activities of extracts from S. dimorphus against bacteria used in this study. Antimicrobial properties of diethyl ether and methanol extracts of algae Scenedesmus dimorphus against Gram positive bacterium Micrococcus lutesus and Gram negative becterium, Aeromonas hydrophila were evaluated. The algae was pure cultured and once the required volume and number of cells achieved coli using agar well diffusion method diethylether and hexane extracts of the algae cells were extracted. Antibacterial properties of the extracts were evaluated using well diffusion method. The results showed inhibitory activities of methanol and ether extracts of Scenedesmus dimorphus against M. luteus and A. hydrophila with 11-13 mm and 13-21 mm diameters of inhibition zone, respectively. In case of diethyl ether extracts also the inhibitory activity observed with diamaters of inhibition zone in the range of 9-23.7 mm and 21.6-6.3 mm respectively against M. luteus and A. hydrophila. Gas chromatography mass spectrophotometry was used to identify different substances within diethyl ether and methanol extracts with hydrocarbon and ester with highest perentage among other substances. The results confirmed antimicrobial activities of extracts from S. dimorphus against bacteria used in this study.

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