University of Guilan Aquatic Animals Nutrition 2980-8499 5 2 2020 02 20 Effects of different mushroom levels on immunity indices of tilapia (Oreochromis niloticus) exposed to chlorpyrifos in experimental condition Effects of different mushroom levels on immunity indices of tilapia (Oreochromis niloticus) exposed to chlorpyrifos in experimental condition 61 70 4172 10.22124/janb.2020.15425.1080 FA Atefeh Iri Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran Seyyed Aliakbar Hedayati Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran Hamed Paknejad Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran Tahereh Bagheri Offshore Water Research Center, Iranian Fisheries Science Research Institute, Agricultural Research, Education and Extension Organization, Chabahar, Sistan and Baluchistan, Iran Seyed Reza Khaleghi Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran Journal Article 2019 04 14  <br /> <strong>Abstract</strong><br /> The aim of this study was to investigate the prebiotic effect of mushroom on the immune indices of tilapia mucus exposed to chlorpyrifos on 120 juveniles tilapia with an average weight of 20 g. So, 4 treatments were considered as (1) control without prebiotics, (2) treatments containing 0.05%, (3) 0.1% and (4) 0.2% of dietary prebiotics. At the end of the feeding period, fish were exposed to chlorpyrifos toxin at a concentration of 0.05 ppm for 14 days and mucus sampling was taken from the fish skin. Experimental treatments exhibited significant effects on alkaline phosphatase, lysozyme, soluble protein, and mucus immunoglobulin (p<0.05). So that, the amounts of alkaline phosphatase, lysozyme and mucus soluble protein were significantly increased in treatments fed with prebiotic-supplemented diets by increasing in prebiotic concentration (40.40 IU/L, 36 Unit/mg Pro and 99.54 µg/100 µL, respectively). However, the amount of mucosal immunoglobulin decreased in these treatments (69 µg/100 µL). Overall, supplementing diets with prebiotic (mushroom powder), leads to enhanced immune system (elevating alkaline phosphatase, lysozyme, immunoglobulin, and soluble protein) when exposed pollutants and stressors.  <br /> <strong>Abstract</strong><br /> The aim of this study was to investigate the prebiotic effect of mushroom on the immune indices of tilapia mucus exposed to chlorpyrifos on 120 juveniles tilapia with an average weight of 20 g. So, 4 treatments were considered as (1) control without prebiotics, (2) treatments containing 0.05%, (3) 0.1% and (4) 0.2% of dietary prebiotics. At the end of the feeding period, fish were exposed to chlorpyrifos toxin at a concentration of 0.05 ppm for 14 days and mucus sampling was taken from the fish skin. Experimental treatments exhibited significant effects on alkaline phosphatase, lysozyme, soluble protein, and mucus immunoglobulin (p<0.05). So that, the amounts of alkaline phosphatase, lysozyme and mucus soluble protein were significantly increased in treatments fed with prebiotic-supplemented diets by increasing in prebiotic concentration (40.40 IU/L, 36 Unit/mg Pro and 99.54 µg/100 µL, respectively). However, the amount of mucosal immunoglobulin decreased in these treatments (69 µg/100 µL). Overall, supplementing diets with prebiotic (mushroom powder), leads to enhanced immune system (elevating alkaline phosphatase, lysozyme, immunoglobulin, and soluble protein) when exposed pollutants and stressors. https://janb.guilan.ac.ir/article_4172_1ea01fe54996e2b48d2b4e4405c91096.pdf