University of Guilan Aquatic Animals Nutrition 2980-8499 5 2 2020 02 20 Reproductive performance improvement of female Zebrafish (Danio rerio) fed with diet contacting lactoferrin and/or spirulina powder Reproductive performance improvement of female Zebrafish (Danio rerio) fed with diet contacting lactoferrin and/or spirulina powder 131 141 4254 10.22124/janb.2020.16631.1089 FA Sanaz Sadeghi Goghari Department of Fisheries, Faculty of Natural Resources, Isfahan University of Technology, Isfahan, Iran Fatemeh Paykan Heyrati Department of Fisheries, Faculty of Natural Resources, Isfahan University of Technology, Isfahan, Iran Salar Dorafshan Department of Fisheries, Faculty of Natural Resources, Isfahan University of Technology, Isfahan, Iran Journal Article 2019 05 23 In this study, the effects of lactoferrin (Lf)and spirulina powder (SP) alone or in combination on reproductive performance of female zebrafish <em>Danio rerio</em> were investigated. The fish with average initial weight of 0.27 g were fed with the basal diet (control, C) or diet enriched with Spirulina powder (SP 100 g/kg, T_SP100) or lactoferrin (Lf 400 mg/kg, T_LF400) or their combination (100 g/kg spirulina and 400 mg/kg lactoferrin, T_{SP100 +} _LF400) for 3 weeks. At the end of the experiment, some reproductive indices were determined including fecundity, egg diameter, hatching and fertilization rates, individual larval length and weight. The highest spawning rate was observed in T_LF400and T_{SP100 +} _LF400. Some reproductive indices such as fertilization and hatching rates were enhanced in T_{SP100 +} _LF400 (p<0.05). Egg diameters, larval survival and weight were also enhanced in T_{SP100 +} _LF400 (p<0.05). In case of the brood fish, which failed to spawn, significant enhancement in gonadosomatic index and relative fecundity were observed only in T_LF400, while absolute fecundity, oocyte diameter and weight were significantly enhanced in T_{SP100 +} _LF400 (p<0.05). Raised reproductive performance in fish fed with lactoferrin and spirulina in this study, indicated their synergism effects on zebrafish diet. In this study, the effects of lactoferrin (Lf)and spirulina powder (SP) alone or in combination on reproductive performance of female zebrafish <em>Danio rerio</em> were investigated. The fish with average initial weight of 0.27 g were fed with the basal diet (control, C) or diet enriched with Spirulina powder (SP 100 g/kg, T_SP100) or lactoferrin (Lf 400 mg/kg, T_LF400) or their combination (100 g/kg spirulina and 400 mg/kg lactoferrin, T_{SP100 +} _LF400) for 3 weeks. At the end of the experiment, some reproductive indices were determined including fecundity, egg diameter, hatching and fertilization rates, individual larval length and weight. The highest spawning rate was observed in T_LF400and T_{SP100 +} _LF400. Some reproductive indices such as fertilization and hatching rates were enhanced in T_{SP100 +} _LF400 (p<0.05). Egg diameters, larval survival and weight were also enhanced in T_{SP100 +} _LF400 (p<0.05). In case of the brood fish, which failed to spawn, significant enhancement in gonadosomatic index and relative fecundity were observed only in T_LF400, while absolute fecundity, oocyte diameter and weight were significantly enhanced in T_{SP100 +} _LF400 (p<0.05). Raised reproductive performance in fish fed with lactoferrin and spirulina in this study, indicated their synergism effects on zebrafish diet. https://janb.guilan.ac.ir/article_4254_e5f8e73f6632070ea7813c3dfea01bb1.pdf