University of Guilan Aquatic Animals Nutrition 2980-8499 7 1 2021 03 21 Dietary effects of lemon Citrus limon peel essential oil encapsulated in chitosan nanoparticles on hematological indices and antioxidant defense system of rainbow trout Oncorhynchus mykiss Dietary effects of lemon Citrus limon peel essential oil encapsulated in chitosan nanoparticles on hematological indices and antioxidant defense system of rainbow trout Oncorhynchus mykiss 27 41 5153 10.22124/janb.2021.20348.1151 FA Abbas Ghytasi Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran Seyed Pezhman Hosseini Shekarabi Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran Houman Rajabi Islami Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran Mehdi Shamsaie Mehrgan Department of Fisheries Science, Science and Research Branch, Islamic Azad University, Tehran, Iran Journal Article 2020 10 16 This study was conducted to evaluate the effect of dietary levels of lemon essential oil encapsulated in chitosan nanoparticles on hematological indices and antioxidant status of rainbow trout fry. Accordingly, the fish (4.74 ± 0.14 g in weight) were distributed in 12 fiberglass tanks with 20 fish per tank and were fed with different levels of lemon essential oil loaded in chitosan nanoparticles including control group with no additive (C_0%), 0.5% (T_0.5%), 0.75% (T_0.75%) and 1% (T_1%) for 8 weeks in triplicates. The results showed that the highest number of RBC was in T_0.75% compared to T_0.5%and C_0% (p<0.05). The maximum hemoglobin value was observed in T_1% and T_0.75%compared to the other experimental groups (p<0.05). In addition, in terms of the blood indices, MCH index showed a significant increase in T_1% and T_0.75%compared to the other treatments. The maximum WBC and lymphocytes were observed in T_1% compared to the other experimental groups (p<0.05). Serum antioxidant enzymes showed the highest catalase activity and glutathione peroxidase in T_1% compared to the other groups (p<0.05). Furthermore, superoxide dismutase activity elevated significantly only in T_1% compared to C_0% (p<0.05). However, the oral administration of 0.75% and 1% of lemon peel essential encapsulated with nanochitosan resulted in a significant elevation in the serum malondialdehyde compared to T_0.5% and C_0%(p<0.05). Overall, the results of this study showed that the addition of lemon essential oil encapsulated in chitosan nanoparticles, especially at 1%, can enhance the hematological indices and antioxidant defense system of rainbow trout. This study was conducted to evaluate the effect of dietary levels of lemon essential oil encapsulated in chitosan nanoparticles on hematological indices and antioxidant status of rainbow trout fry. Accordingly, the fish (4.74 ± 0.14 g in weight) were distributed in 12 fiberglass tanks with 20 fish per tank and were fed with different levels of lemon essential oil loaded in chitosan nanoparticles including control group with no additive (C_0%), 0.5% (T_0.5%), 0.75% (T_0.75%) and 1% (T_1%) for 8 weeks in triplicates. The results showed that the highest number of RBC was in T_0.75% compared to T_0.5%and C_0% (p<0.05). The maximum hemoglobin value was observed in T_1% and T_0.75%compared to the other experimental groups (p<0.05). In addition, in terms of the blood indices, MCH index showed a significant increase in T_1% and T_0.75%compared to the other treatments. The maximum WBC and lymphocytes were observed in T_1% compared to the other experimental groups (p<0.05). Serum antioxidant enzymes showed the highest catalase activity and glutathione peroxidase in T_1% compared to the other groups (p<0.05). Furthermore, superoxide dismutase activity elevated significantly only in T_1% compared to C_0% (p<0.05). However, the oral administration of 0.75% and 1% of lemon peel essential encapsulated with nanochitosan resulted in a significant elevation in the serum malondialdehyde compared to T_0.5% and C_0%(p<0.05). Overall, the results of this study showed that the addition of lemon essential oil encapsulated in chitosan nanoparticles, especially at 1%, can enhance the hematological indices and antioxidant defense system of rainbow trout. https://janb.guilan.ac.ir/article_5153_aefb58329d5026eb738f8c9f1b347f19.pdf