University of Guilan Aquatic Animals Nutrition 2980-8499 3 2 2017 09 23 Determination of aluminium bioaccumulation in liver, gill and muscle tissues of the common carp (Cyprinus carpio) Determination of aluminium bioaccumulation in liver, gill and muscle tissues of the common carp (Cyprinus carpio) 1 12 3163 10.22124/janb.2018.3163 FA Mohadeseh Miri Natural Ecosystems Management Department, Hamoun International Wetland Research Institute, University of Zabol, Zabol, Sistan & Baluchistan, Iran Hashem Khandan Barani Fisheries Department, Hamoun International Wetland Research Institute, University of Zabol, Zabol, Sistan & Baluchistan, Iran Journal Article 2017 02 08 Aluminium is an abundant metal on earth and its concentration has increased in aquatic environments as a result of natural and industrial activities. The aim of this study was to examine the bioaccumulation of aluminium in tissues of liver, gill and muscle of common carp (Cyprinus carpio) that was used in concentrations of 1, 2, 4 and 6 mg/L in vitro. The metal in the tissues after chemical digestion was measured by atomic absorption spectrophotometer. Compare the amount of aluminium accumulation in the tissues of gill, liver and muscle showed significant differences in different concentrations of aluminium with the control group (P<0.05). The highest amount of aluminium accumulation in gill, muscle and liver were 51.3 ± 0.4, 20.86 ± 0.5 and 11.50 ± 0.28 μg/g D.W at a concentration of 6 mg (96 hours), respectively, and the lowest were 1.2 ± 0.01, 2.9 ± 0.1 and 0.18 ± 0.3 μg/g D.W in the control group. The results showed that the highest bioaccumulation of aluminium in different tissues were gill, liver and muscle, respectively. Also, it was found that with increasing concentration of aluminium in the environment, bio-accumulation index decreased because of probably physiological activity of tissues. Significant relationship was observed between exposed time and aluminium accumulation in muscle and liver tissue. The gill is the target organ for aluminium that can be used as an indicator of environmental contamination. Aluminium is an abundant metal on earth and its concentration has increased in aquatic environments as a result of natural and industrial activities. The aim of this study was to examine the bioaccumulation of aluminium in tissues of liver, gill and muscle of common carp (Cyprinus carpio) that was used in concentrations of 1, 2, 4 and 6 mg/L in vitro. The metal in the tissues after chemical digestion was measured by atomic absorption spectrophotometer. Compare the amount of aluminium accumulation in the tissues of gill, liver and muscle showed significant differences in different concentrations of aluminium with the control group (P<0.05). The highest amount of aluminium accumulation in gill, muscle and liver were 51.3 ± 0.4, 20.86 ± 0.5 and 11.50 ± 0.28 μg/g D.W at a concentration of 6 mg (96 hours), respectively, and the lowest were 1.2 ± 0.01, 2.9 ± 0.1 and 0.18 ± 0.3 μg/g D.W in the control group. The results showed that the highest bioaccumulation of aluminium in different tissues were gill, liver and muscle, respectively. Also, it was found that with increasing concentration of aluminium in the environment, bio-accumulation index decreased because of probably physiological activity of tissues. Significant relationship was observed between exposed time and aluminium accumulation in muscle and liver tissue. The gill is the target organ for aluminium that can be used as an indicator of environmental contamination.

https://janb.guilan.ac.ir/article_3163_1dca54fc49d2880d8c4e0c835b325207.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 3 2 2017 09 23 Changes in body composition of western white leg shrimp (Litopenaeus vannamei) at different stages of growth Changes in body composition of western white leg shrimp (Litopenaeus vannamei) at different stages of growth 13 18 3164 10.22124/janb.2018.3164 FA Ghais Koti Department of Aquaculture, College of Agriculture and Natural Resources, Ahvaz Branch, Islamic Azad University, Ahvaz, Khuzestan, Iran Abolfazl Askary Sary Department of Fisheries, College of Agriculture and Natural Resources, Ahvaz Branch, Islamic Azad University, Ahvaz, Khuzestan, Iran Mohammad Velayatzadeh Young Researchers and Elite Club, Ahvaz Branch, Islamic Azad University, Ahvaz, Khuzestan, Iran Journal Article 2017 07 01 The study of changes the amount of protein, lipid, carbohydrates, fiber, ash and moisture of farmed Litopenaeus vannamei at different stages of growth was performed. Samples at the stages of post larvae 12, shrimp with 1, 2 and 3 months age, grow-out and market shrimp at the end of the period were provided from hatchery and breeding centers in Khuzestan province. Chemical composition w measured by standard methods (AOAC) in muscle of L. vannamei. The highest moisture level in shrimp with 1 month age was 78.3% and the lowest in the market shrimp was 71.43% (P<0.05). The highest protein in muscle of shrimp was 20.18 % and the lowest was in post larvae 12, 15% (P<0.05). The highest lipid in muscle of shrimp was 0.83% and the lowest was 0.4% at stage of 12 post larvae (P<0.05). The highest of carbohydrate in post larvae 12 was 5.73% and the lowest in market shrimp was 0.14%. The highest amount of fiber (0.23%) was measured in 3 months age shrimp (P>0.05). According to the results, by the time that L. vannamei grows, the amount of protein in the muscles increases and this feature makes it suitable for human consumption. The study of changes the amount of protein, lipid, carbohydrates, fiber, ash and moisture of farmed Litopenaeus vannamei at different stages of growth was performed. Samples at the stages of post larvae 12, shrimp with 1, 2 and 3 months age, grow-out and market shrimp at the end of the period were provided from hatchery and breeding centers in Khuzestan province. Chemical composition w measured by standard methods (AOAC) in muscle of L. vannamei. The highest moisture level in shrimp with 1 month age was 78.3% and the lowest in the market shrimp was 71.43% (P<0.05). The highest protein in muscle of shrimp was 20.18 % and the lowest was in post larvae 12, 15% (P<0.05). The highest lipid in muscle of shrimp was 0.83% and the lowest was 0.4% at stage of 12 post larvae (P<0.05). The highest of carbohydrate in post larvae 12 was 5.73% and the lowest in market shrimp was 0.14%. The highest amount of fiber (0.23%) was measured in 3 months age shrimp (P>0.05). According to the results, by the time that L. vannamei grows, the amount of protein in the muscles increases and this feature makes it suitable for human consumption.

https://janb.guilan.ac.ir/article_3164_175cdf38f5da37dc58217a2315b8fdac.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 3 2 2017 09 23 Individual and combined effects of nisin-Z and sodium citrate on the chemical attributes and lactic acid, mesophyll and psychrophill bacteria communities of Caspian Kutum fillets (Rutilus frisii kutum) Stored at 4 ºC Individual and combined effects of nisin-Z and sodium citrate on the chemical attributes and lactic acid, mesophyll and psychrophill bacteria communities of Caspian Kutum fillets (Rutilus frisii kutum) Stored at 4 ºC 19 34 2242 FA Masoud Hedayatifard Department of Fisheries, College of Natural Resources, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Mazandaran, Iran Mojgan Alinezhad Alinezhad Department of Fisheries, Advanced Educational Center, Qaemshahr Branch, Islamic Azad University, Qaemshahr, Mazandaran, Iran Masoud Hashemi Karouei Department of Veterinary, College of Veterinary, Babol Branch, Islamic Azad University, Babol, Mazandaran, Iran Journal Article 2017 06 29 Individual and combined effectsof Nisin Z (1.5%) and sodium citrate (2%) in air-packed Caspian Kutum fillets during the refrigerated storage at 4°C was investigated. Changes in some quality indexes of fish including chemical attributes including TVB-N, PV and TBA and microbial communities counts including mesophilic (TMC), psychrophill (TPC) and lactic acid bacteria (LAB) were determined during days of 0, 3, 6, 9 and 12. The results showed that TVB-N, PV and TBA indices in treatments were higher than control. Mesophilic and psychrophill bacteria in control sample (with 6.53 and 6.71 CFU/g, respectively) reached to acceptable amount, while treatments containing preservatives showed a better condition in both TPC (with 6.63 to 6.93 CFU/g) and TMC (with 6.67 to 6.90 CFU/g) (P<0.01). Also, growth of LAB has controlled at 9 days (with 6.88 CFU/g) but stopped in treatment samples at the same days (P<0.01). The results revealed that Nisin Z in combination with sodium citrate can therefore be used as the effective preservatives to shelf-life of Caspian Kutum fillets during refrigerated storage. Individual and combined effectsof Nisin Z (1.5%) and sodium citrate (2%) in air-packed Caspian Kutum fillets during the refrigerated storage at 4°C was investigated. Changes in some quality indexes of fish including chemical attributes including TVB-N, PV and TBA and microbial communities counts including mesophilic (TMC), psychrophill (TPC) and lactic acid bacteria (LAB) were determined during days of 0, 3, 6, 9 and 12. The results showed that TVB-N, PV and TBA indices in treatments were higher than control. Mesophilic and psychrophill bacteria in control sample (with 6.53 and 6.71 CFU/g, respectively) reached to acceptable amount, while treatments containing preservatives showed a better condition in both TPC (with 6.63 to 6.93 CFU/g) and TMC (with 6.67 to 6.90 CFU/g) (P<0.01). Also, growth of LAB has controlled at 9 days (with 6.88 CFU/g) but stopped in treatment samples at the same days (P<0.01). The results revealed that Nisin Z in combination with sodium citrate can therefore be used as the effective preservatives to shelf-life of Caspian Kutum fillets during refrigerated storage.

https://janb.guilan.ac.ir/article_2242_780c83fedf60200df5bdd6e5f29c228a.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 3 2 2017 09 23 Anesthetic effects and biochemical changes of peppermint essence (Mentha spicata) in snow trout (Schizothorax zarudnyi) Anesthetic effects and biochemical changes of peppermint essence (Mentha spicata) in snow trout (Schizothorax zarudnyi) 35 46 2238 FA Abdolali Rahdari Department of Fisheries, International Hamun Wetland Institute, University of Zabol, Zabol, Sistan & Baluchistan, Iran Ali Khosravanizadeh Department of Fisheries, International Hamun Wetland Institute, University of Zabol, Zabol, Sistan & Baluchistan, Iran Hosein Dahmardeh Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Sistan & Baluchistan, Iran Ahmad Gharaei Department of Fisheries, International Hamun Wetland Institute, University of Zabol, Zabol, Sistan & Baluchistan, Iran Javad Mirdar Harijani Department of Fisheries, Faculty of Natural Resources, University of Zabol, Zabol, Sistan & Baluchistan, Iran Journal Article 2017 04 04 By increasing the aquaculture facilities, using effective anesthetics and introducing new ingredients is so important. In this study,the efficacy of peppermint (Menthaspicata) as an anesthetic for snow trout (Schizothorax zarudnyi) was investigated. The peppermint essence was used for 30 farmed snow trout with mean (±SD) weight 90.97 ± 38.26 g. The fish were divided into five treatments (each consisting of six fish). Four treatments were exposed to peppermint with 200, 250, 350 and 500 mg L-1 concentrations by immersion method. The control group was not exposed to any dose of the anesthetic. The mean time of each anesthetic doses and recovery stages were measured. Results showed that by increasing the peppermint doses, the time of anesthesia were reduced. The minimum and maximum times of total anesthesia were recorded in 500 and 250 mg L-1 with 161.00 ± 83.20 and 88.17 ± 22.52 seconds, while those in 200 mg L-1 was significantly different from the other treatments. The mean recovery time in snow trout in treatment 200 mg L-1 was the lowest (180.00 ± 97.35s) compared to the other treatments. Acute toxicity test indicated that the 96-h LC50 value of peppermint essencewas 119.58 mg L-1. The result of this experiment clearly indicated that peppermint essence in 200 mg L-1 could be used as an effective anesthetic for handling and transport practices of this species. By increasing the aquaculture facilities, using effective anesthetics and introducing new ingredients is so important. In this study,the efficacy of peppermint (Menthaspicata) as an anesthetic for snow trout (Schizothorax zarudnyi) was investigated. The peppermint essence was used for 30 farmed snow trout with mean (±SD) weight 90.97 ± 38.26 g. The fish were divided into five treatments (each consisting of six fish). Four treatments were exposed to peppermint with 200, 250, 350 and 500 mg L-1 concentrations by immersion method. The control group was not exposed to any dose of the anesthetic. The mean time of each anesthetic doses and recovery stages were measured. Results showed that by increasing the peppermint doses, the time of anesthesia were reduced. The minimum and maximum times of total anesthesia were recorded in 500 and 250 mg L-1 with 161.00 ± 83.20 and 88.17 ± 22.52 seconds, while those in 200 mg L-1 was significantly different from the other treatments. The mean recovery time in snow trout in treatment 200 mg L-1 was the lowest (180.00 ± 97.35s) compared to the other treatments. Acute toxicity test indicated that the 96-h LC50 value of peppermint essencewas 119.58 mg L-1. The result of this experiment clearly indicated that peppermint essence in 200 mg L-1 could be used as an effective anesthetic for handling and transport practices of this species. https://janb.guilan.ac.ir/article_2238_d0685c2241576580644fb26c6c542cab.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 3 2 2017 09 23 Organoleptic assessment of silver carp (Hypophthalmichthys molitrix) fish finger coated by different citrus fruit extracts Organoleptic assessment of silver carp (Hypophthalmichthys molitrix) fish finger coated by different citrus fruit extracts 47 55 2239 FA Seyed Vali Hosseini Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Alborz, Iran Farzaneh Sayadi Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Alborz, Iran Kobra Ziyaei Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Alborz, Iran Journal Article 2017 05 01 Nowadays, employing natural compounds has been increased to improve organoleptic properties of fisheries products. In this study, we evaluated the effects of coating by citrus fruits extracts (orange, bitter orange and lime) in different concentrations (0, 5, 10, and 20% of coated materials) on the organoleptic properties of silver carp (Hypophthalmichthys molitrix) fish finger (SCFF). SCFFs were steam - cooked (20 min at 180°C) and then the organoleptic properties were evaluated by semi-trained panelists. Results showed that SCFFs coated with orange extract, especially at 20% concentration, was better than the other treatments in term of odor index (P<0.05). Color and taste values showed higher scores for those coated with 10 and 20% lime extracts. Furthermore, those with 20% lime extract exhibited the best flavor compared to the other treatments. From total acceptability perspective, there was no significant difference between 10 and 20% in all experimental citrus fruits extracts. According to the results, employing 10% citrus fruit extracts could be the proper level for the preparation of SCFF citrus coating. On the other hand, because the three type of citrus fruit selected are in the same family, so we can use them in the fish fingers or analog products on the base of their availability and price. Nowadays, employing natural compounds has been increased to improve organoleptic properties of fisheries products. In this study, we evaluated the effects of coating by citrus fruits extracts (orange, bitter orange and lime) in different concentrations (0, 5, 10, and 20% of coated materials) on the organoleptic properties of silver carp (Hypophthalmichthys molitrix) fish finger (SCFF). SCFFs were steam - cooked (20 min at 180°C) and then the organoleptic properties were evaluated by semi-trained panelists. Results showed that SCFFs coated with orange extract, especially at 20% concentration, was better than the other treatments in term of odor index (P<0.05). Color and taste values showed higher scores for those coated with 10 and 20% lime extracts. Furthermore, those with 20% lime extract exhibited the best flavor compared to the other treatments. From total acceptability perspective, there was no significant difference between 10 and 20% in all experimental citrus fruits extracts. According to the results, employing 10% citrus fruit extracts could be the proper level for the preparation of SCFF citrus coating. On the other hand, because the three type of citrus fruit selected are in the same family, so we can use them in the fish fingers or analog products on the base of their availability and price.

https://janb.guilan.ac.ir/article_2239_f273c22026862eda10812a248411052d.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 3 2 2017 09 23 Comparison of fatty acids biochemical quality in the caviar of reared and wild beluga, Huso huso Comparison of fatty acids biochemical quality in the caviar of reared and wild beluga, Huso huso 57 67 2240 FA Seyed Vali Hosseini Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Alborz, Iran Aria Babakhani Lashkan Babakhani Lashkan Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran Amin Oji Fard Fisheries Department, Faculty of Agriculture and Natural Resources, Persian Gulf University, Borazjan, Bushehr, Iran Journal Article 2017 05 01 In this study, the quality of fatty acids in sturgeon caviar were assessed and compared in rearedand wild specimens of beluga, Huso huso caught from the Caspian Sea. The fatty acids profile, n3/n6 and atherogenic and thrombogenic index were also measured. The wild caviar had higher PUFA and n3/n6 than reared fish (P<0.05). The EPA + DHA content in wild caviar (20.7) was significantly higher than reared specimens (10.54) (P<0.05). Atherogenic index in wild (0.33) was significantly higher than reared (0.27), while thrombogenic index in reared was higher than wild (P<0.05). The results obtained from the present study show that the reared beluga caviar has a good quality and is a suitable alternative for wild caviar. In this study, the quality of fatty acids in sturgeon caviar were assessed and compared in rearedand wild specimens of beluga, Huso huso caught from the Caspian Sea. The fatty acids profile, n3/n6 and atherogenic and thrombogenic index were also measured. The wild caviar had higher PUFA and n3/n6 than reared fish (P<0.05). The EPA + DHA content in wild caviar (20.7) was significantly higher than reared specimens (10.54) (P<0.05). Atherogenic index in wild (0.33) was significantly higher than reared (0.27), while thrombogenic index in reared was higher than wild (P<0.05). The results obtained from the present study show that the reared beluga caviar has a good quality and is a suitable alternative for wild caviar.

https://janb.guilan.ac.ir/article_2240_e23c7b05ceb9f1f1de4774964dea6022.pdf