University of Guilan Aquatic Animals Nutrition 2980-8499 6 2 2020 06 21 Effects of Lavandula angostifolia on growth performance, survival rate and immunity system in Grass carp (Ctenopharyngodon idella) Effects of Lavandula angostifolia on growth performance, survival rate and immunity system in Grass carp (Ctenopharyngodon idella) 1 12 4586 10.22124/janb.2021.18059.1112 FA Raha Fadaei Raieni Department of Fisheries Science and Engineering, Faculty of Natural Resources, University of Jiroft, Jiroft, Kerman, Iran Taiebeh Enaiat Gholampor Dezful Fisheries Office, Khuzestan General Fisheries Office, Iran Fisheries Organization, Dezful, Khuzestan, Iran Javad Sepahi Department of Plant Production, Faculty of Agriculture, Saravan Integrated Education, Saravan, Sistan and Baluchistan, Iran Journal Article 2020 01 31 The Lavandula angostifolia is a medicinal plant that has the property of strengthening the immune system. In the present study, the effects of different levels of L. angostifolia extract on growth performance, survival rate and blood indices of the grass carp (Ctenopharyngodon idella) (11.01 ± 0.05 g, 15.5 ± 0.3 cm) was investigated. The fish were didtributed in 4 treatments (0 (control), 0.1% (T1), 0.5% (T2) and 1% (T3)). The fish were fed for 8 weeks. The results showed highest and lowest weight in both leaves and lavender groups in T3 and control group, respectively (p < 0.05). The highest and lowest FCR were observed in the control group and T3 treatment, respectively (p < 0.05). WG, BWI and PER values in T3 had the highest value (p < 0.05). CF in treatments containing common lavender was higher than control (p < 0.05). There was no significant difference in HSI (p>0.05). The highest and lowest HSI was observed in control and T2 groups. No mortality was observed in the groups. The hematocrit, hemoglobin, RBC, WBC had significant difference (p < 0.05) but lymphocytes, monocytes, neutrophils, MCH, MCV and MCHC showed no significant difference (p>0.05). The levels of lysozyme, IgM and globulin in experimental treatments were significantly higher than control (p < 0.05). In general, the extract of common lavender has a positive effect on improving the biological status of grass carp. The Lavandula angostifolia is a medicinal plant that has the property of strengthening the immune system. In the present study, the effects of different levels of L. angostifolia extract on growth performance, survival rate and blood indices of the grass carp (Ctenopharyngodon idella) (11.01 ± 0.05 g, 15.5 ± 0.3 cm) was investigated. The fish were didtributed in 4 treatments (0 (control), 0.1% (T1), 0.5% (T2) and 1% (T3)). The fish were fed for 8 weeks. The results showed highest and lowest weight in both leaves and lavender groups in T3 and control group, respectively (p < 0.05). The highest and lowest FCR were observed in the control group and T3 treatment, respectively (p < 0.05). WG, BWI and PER values in T3 had the highest value (p < 0.05). CF in treatments containing common lavender was higher than control (p < 0.05). There was no significant difference in HSI (p>0.05). The highest and lowest HSI was observed in control and T2 groups. No mortality was observed in the groups. The hematocrit, hemoglobin, RBC, WBC had significant difference (p < 0.05) but lymphocytes, monocytes, neutrophils, MCH, MCV and MCHC showed no significant difference (p>0.05). The levels of lysozyme, IgM and globulin in experimental treatments were significantly higher than control (p < 0.05). In general, the extract of common lavender has a positive effect on improving the biological status of grass carp.

https://janb.guilan.ac.ir/article_4586_93f8b1dd70d3df269fade70bbb11d68a.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 6 2 2020 06 21 Effects of microencapsulated Lactobacillus rhamnosus JCM 1136 probiotics on blood parameters and body composition of rainbow trout Effects of microencapsulated Lactobacillus rhamnosus JCM 1136 probiotics on blood parameters and body composition of rainbow trout 13 25 4601 10.22124/janb.2021.18496.1117 FA Amir Veisi Department of Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran Hossein Ouraji Department of Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran Farid Firouzbakhsh Department of Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran Gholamreza Rafiee Department of Fisheries, Campus of Agriculture and Natural Resources, University of Tehran, Faculty of Natural Resources, Karaj, Alborz, Iran Abdolsamad Keramat Department of Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran Journal Article 2019 04 04 Effects of the microencapsulated Lactobacillus rhamnosus JCM 1136 on the blood parameters and body composition of rainbow trout (Oncorhynchus mykiss). In this study, first the probiotic bacteria L. rhamnosus JCM1136 were microencapsulated with coatings of sodium alginate and chitosan. Then, they were added to the food pellets and given to the fingerlings. For this purpose, 225 rainbow trout fingerlings (6.23 ± 0.17 g) in 5 treatments and each treatment with 3 replications were placed in California trays (220 × 30 × 15 cm). Treatment 1 (T1): Fish fed with 108 CFU/g microencapsulated probiotics with sodium alginate, treatment 2 (T2): fish fed with 108 CFU/g microencapsulated probiotics with sodium alginate and chitosan, treatment 3 (T3): fish fed with 108 CFU/g capsule-free probiotics, treatment 4 (T4): fish fed with probiotic-free sodium alginate-chitosan capsules and control treatment 5 (T5): fish fed with commercial free-probiotic and capsules pellets. At the end of the course, results indicated that the fishes fed with diet containing sodium alginate-chitosan microencapsulated probiotics in terms of blood parameters such as red blood cells, white blood cells, hemoglobin and hematocrit as well as the amount of carcass protein compared to control and other treatments were in better condition, which These differences were significant with control treatments fishes (p < 0.05). Effects of the microencapsulated Lactobacillus rhamnosus JCM 1136 on the blood parameters and body composition of rainbow trout (Oncorhynchus mykiss). In this study, first the probiotic bacteria L. rhamnosus JCM1136 were microencapsulated with coatings of sodium alginate and chitosan. Then, they were added to the food pellets and given to the fingerlings. For this purpose, 225 rainbow trout fingerlings (6.23 ± 0.17 g) in 5 treatments and each treatment with 3 replications were placed in California trays (220 × 30 × 15 cm). Treatment 1 (T1): Fish fed with 108 CFU/g microencapsulated probiotics with sodium alginate, treatment 2 (T2): fish fed with 108 CFU/g microencapsulated probiotics with sodium alginate and chitosan, treatment 3 (T3): fish fed with 108 CFU/g capsule-free probiotics, treatment 4 (T4): fish fed with probiotic-free sodium alginate-chitosan capsules and control treatment 5 (T5): fish fed with commercial free-probiotic and capsules pellets. At the end of the course, results indicated that the fishes fed with diet containing sodium alginate-chitosan microencapsulated probiotics in terms of blood parameters such as red blood cells, white blood cells, hemoglobin and hematocrit as well as the amount of carcass protein compared to control and other treatments were in better condition, which These differences were significant with control treatments fishes (p < 0.05).

https://janb.guilan.ac.ir/article_4601_2c31d9fc43d630da36659dbe1c01fcc5.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 6 2 2020 06 21 A survey on growth indices and intestinal enzymes activity affected by feeding Beluga juveniles (Huso huso) subjected to a diet containing different levels of dietary -sodium chloride (NaCl) A survey on growth indices and intestinal enzymes activity affected by feeding Beluga juveniles (Huso huso) subjected to a diet containing different levels of dietary -sodium chloride (NaCl) 27 38 4614 10.22124/janb.2021.17393.1102 FA Sommayeh Hasanpour Golden Caviar Breeding Farm, Sangar Dam, Rasht, Guilan, Iran Mohammad Pourdehghani International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Guilan, Iran Habib Vahabzadeh Department of Fisheries, Lahijan Branch, Islamic Azad University, Lahijan, Guilan, Iran Mamoud Mohseni International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Guilan, Iran Amin Zarabi Shohaday-e-Kheibar Abkenar Sturgeon Rearing and Propagation Complex, Guilan, Iran Journal Article 2020 04 02 The current study carried out to investigate the effects of adding salt (NaCl) in the diet of beluga, Huso huso and its impacts both on the function of intestinal enzymes and growth indices and food efficiency. In this regard, 480 species of Huso huso with a mean weight of 173.8 ± 0.49 g stocked in 12 fiberglasses 2000-liter tanks for 12 weeks. They fed a diet containing different levels of NaCl including 0, 3, 6 and 9 percent. Growth indices including final weight, SGR and daily growth showed significant differences in fishes of all treatments, except the fishes fed the diet containing comparing control group (p < 0.05) Weight gain percentage of fed fish at 6% sodium chloride level was significantly higher than fish fed control but no statistically significant difference was observed between other treatments. The mean FCR in fishes of control group was significantly higher than other treatments (p < 0.05). The mean rates ofrate of intestinal enzymes including Alkaline phosphatase, protease, lipase and amylase in the fishes fed by different levels of NaCl were significantly more than control group (p < 0.05). It is suggested to achieve a higher growth rate, improve food efficiency, reduction of production costs and enhancement of intestinal enzymes function. appropriate percentage of added NaCl is to be 3-6% per kilogram of diet for farmed beluga of at 150 to 700 g. The current study carried out to investigate the effects of adding salt (NaCl) in the diet of beluga, Huso huso and its impacts both on the function of intestinal enzymes and growth indices and food efficiency. In this regard, 480 species of Huso huso with a mean weight of 173.8 ± 0.49 g stocked in 12 fiberglasses 2000-liter tanks for 12 weeks. They fed a diet containing different levels of NaCl including 0, 3, 6 and 9 percent. Growth indices including final weight, SGR and daily growth showed significant differences in fishes of all treatments, except the fishes fed the diet containing comparing control group (p < 0.05) Weight gain percentage of fed fish at 6% sodium chloride level was significantly higher than fish fed control but no statistically significant difference was observed between other treatments. The mean FCR in fishes of control group was significantly higher than other treatments (p < 0.05). The mean rates ofrate of intestinal enzymes including Alkaline phosphatase, protease, lipase and amylase in the fishes fed by different levels of NaCl were significantly more than control group (p < 0.05). It is suggested to achieve a higher growth rate, improve food efficiency, reduction of production costs and enhancement of intestinal enzymes function. appropriate percentage of added NaCl is to be 3-6% per kilogram of diet for farmed beluga of at 150 to 700 g.

https://janb.guilan.ac.ir/article_4614_91d22817683cc3abc1a45ae69a65e1b8.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 6 2 2020 06 21 Effects of different levels of dietary taurine amino acid on Rosy Barb (Pethia conchonius) pigmentation Effects of different levels of dietary taurine amino acid on Rosy Barb (Pethia conchonius) pigmentation 39 48 4637 10.22124/janb.2021.16792.1091 FA Fereshteh Nejatizadegan Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran Fatemeh Paykan Heyrati Department of Natural Resources, Isfahan University of Technology, Isfahan, Iran Salar Dorafshan Department of Natural Resources, Isfahan University of technology, Isfahan, Iran Vahid Morshedi Department of Natural Resources, Persian Gulf University, Bushehr, Iran Journal Article 2020 04 16 Taurine (2 amino ethane sulfonic acid), is a semi-essential amino acid and an end product of the sulfur amino acid metabolism. The aim of this study was improving Rosy barb (Pethia conchonius) pigmentation. The fish (average weight 32 ± 7 mg and length 12.83 ± 1.82 mm) were divided into five different groups including control (basic diet without taurine and astaxanthin) and four different levels of taurine (0, 100, 250 & 500 mg/kg diet) with constant amount of astaxanthin (100 mg/kg diet) and fed for 60 days. The pigmentation analysis was done using digital camera photography and whole body biochemical carotenoid analysis and showed that the fish fed on 100 + 250 mg/kg of astaxanthin & taurine respectively had the highest levels of pigmentation. Adding taurine up to 250 (mg/kg diet) caused increasing total carcasses lipid, plasma triglyceride, cholesterol, LDL & HDL levels in compression to the fish fed on control diet (p<0.05). It could be concluded that dietary taurine supplementation could affect fish lipid metabolism and improve astaxanthin absorption, which lead to better pigmentation in Rosy barb. Taurine (2 amino ethane sulfonic acid), is a semi-essential amino acid and an end product of the sulfur amino acid metabolism. The aim of this study was improving Rosy barb (Pethia conchonius) pigmentation. The fish (average weight 32 ± 7 mg and length 12.83 ± 1.82 mm) were divided into five different groups including control (basic diet without taurine and astaxanthin) and four different levels of taurine (0, 100, 250 & 500 mg/kg diet) with constant amount of astaxanthin (100 mg/kg diet) and fed for 60 days. The pigmentation analysis was done using digital camera photography and whole body biochemical carotenoid analysis and showed that the fish fed on 100 + 250 mg/kg of astaxanthin & taurine respectively had the highest levels of pigmentation. Adding taurine up to 250 (mg/kg diet) caused increasing total carcasses lipid, plasma triglyceride, cholesterol, LDL & HDL levels in compression to the fish fed on control diet (p<0.05). It could be concluded that dietary taurine supplementation could affect fish lipid metabolism and improve astaxanthin absorption, which lead to better pigmentation in Rosy barb.

https://janb.guilan.ac.ir/article_4637_21c75c42199b6632fd8747d72e2b9225.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 6 2 2020 06 21 The effect of chitosan nanoparticles coated with folic acid to feed on growth parameters in rainbow trout (Oncorhynchus mykiss) fingerlings The effect of chitosan nanoparticles coated with folic acid to feed on growth parameters in rainbow trout (Oncorhynchus mykiss) fingerlings 49 62 4701 10.22124/janb.2021.18764.1119 FA Sahar Farahnak Roudsari Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran Seyed Abdolmajid Mousavi Department of Animal Science, Varamin-Pishva Branch, Islamic Azad University, Varamin, Iran Houman Rajabi Islami Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran Mehdi Shamsaei Mehrgan Department of Fisheries, Science and Research Branch, Islamic Azad University, Tehran, Iran Journal Article 2020 03 01 ABSTRACT The aim of this study was to investigate the effect of chitosan nanoparticles containing folic acid in the diet of rainbow trout, Oncorhynchus mykiss on growth and survival indices. For this purpose, rainbow trout with an average weight of 30 ± 2 g were divided into five experimental treatments: 0, 0.1, 0.25, 0.5 and 1 mg of chitosan/folic acid nanoparticles per kg of diet. Each treatment containing 25 fish in 3 repetitions in 15 tanks (500 liter) was designed into a closed-circuit breeding system. After making a manual diet and adapting the fish to the new conditions they were fed for 8 weeks. At the end of the experiment period, growth and survival indices were evaluated. The highest growth rate was recorded in the treatment of 1 mg/kg of chitosan/folic acid nanoparticles at the rate of 69.23 ± 4.34 g. With increasing Ch/FA nanoparticles levels in the formulated diets, the feed conversion ratio decreased linearly, which in fish fed with 1 mg/kg diet supplemented with chitosan / folic acid nanoparticles reached to 1.29 ± 0.08 (p < 0.05). On the other hand, the survival rate was the same in all treatments, but at the end of the period, losses were clearly observed in the control. The results showed that increasing the level of chitosan/folic acid nanoparticles in formulated diets will increase the growth rate and have a positive effect on growth index. ABSTRACT The aim of this study was to investigate the effect of chitosan nanoparticles containing folic acid in the diet of rainbow trout, Oncorhynchus mykiss on growth and survival indices. For this purpose, rainbow trout with an average weight of 30 ± 2 g were divided into five experimental treatments: 0, 0.1, 0.25, 0.5 and 1 mg of chitosan/folic acid nanoparticles per kg of diet. Each treatment containing 25 fish in 3 repetitions in 15 tanks (500 liter) was designed into a closed-circuit breeding system. After making a manual diet and adapting the fish to the new conditions they were fed for 8 weeks. At the end of the experiment period, growth and survival indices were evaluated. The highest growth rate was recorded in the treatment of 1 mg/kg of chitosan/folic acid nanoparticles at the rate of 69.23 ± 4.34 g. With increasing Ch/FA nanoparticles levels in the formulated diets, the feed conversion ratio decreased linearly, which in fish fed with 1 mg/kg diet supplemented with chitosan / folic acid nanoparticles reached to 1.29 ± 0.08 (p < 0.05). On the other hand, the survival rate was the same in all treatments, but at the end of the period, losses were clearly observed in the control. The results showed that increasing the level of chitosan/folic acid nanoparticles in formulated diets will increase the growth rate and have a positive effect on growth index.

https://janb.guilan.ac.ir/article_4701_8a242043f26cc904b4a8b225c8df482b.pdf

University of Guilan Aquatic Animals Nutrition 2980-8499 6 2 2020 06 21 The effect of vitamins E, C and selenium as stimulants of the immune system on blood factors, growth and immunity of Asian cates (Lates calcarifer) The effect of vitamins E, C and selenium as stimulants of the immune system on blood factors, growth and immunity of Asian cates (Lates calcarifer) 63 74 4713 10.22124/janb.2021.17389.1103 FA Mehrdad Mohammadidust Aquaculture Research Center-South of Iran, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran Mina Ahangarzadeh Aquaculture Research Center-South of Iran, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran Hossein Houshmand, Aquaculture Research Center-South of Iran, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran Fatemeh Hekmatpour Aquaculture Research Center-South of Iran, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran Mohammad Khosravi Department of Pathobiology, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Iran Takavar Mohammadian Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran Lefteh Mohseni Aquaculture Research Center-South of Iran, Iranian Fisheries Science Research Institute, Agricultural Research Education and Extension Organization (AREEO), Ahvaz, Iran Journal Article 2020 03 28 ABSTRACT This study was performed to evaluate the effect of immune system stimulants (vitamins E, C and selenium) on biochemical and immunity indices of Late calcarifer. For this purpose, two treatments were selected. Treatment 1 or control group was fed with food without immune stimulant and treatment 2 with diet containing vitamins E, C and selenium at 2% of the biomass weight. Finally, growth efficiency indices were compared in two treatments. Results showed final weight of the control and treatment groups was 761.33 ± 35.8 g and 850.00 ± 5.77 g, respectively. The specific growth rate of the control and treatment groups was 1.3 ± 0.02 and 1.35 ± 0.01 but the feed conversion ratio did not show a significant difference. Hemoglobin and erythrocytes showed a significant increase compared to the control in treatments containing immune stimulants and supplementation (p < 0.05). White blood cells ranged from 41417 ± 2964.84 in the treatment to 42500 ± 683.13 in the control treatment. The number of red blood cells in the control treatment was 2810000 ± 19.1763 and the recipient treatment was 3951667 ± 14588.84. Immune system stimulants including vitamins E, C and selenium can play a role in improving the growth, blood, biochemical and safety indices of farmed Late calcarifer. ABSTRACT This study was performed to evaluate the effect of immune system stimulants (vitamins E, C and selenium) on biochemical and immunity indices of Late calcarifer. For this purpose, two treatments were selected. Treatment 1 or control group was fed with food without immune stimulant and treatment 2 with diet containing vitamins E, C and selenium at 2% of the biomass weight. Finally, growth efficiency indices were compared in two treatments. Results showed final weight of the control and treatment groups was 761.33 ± 35.8 g and 850.00 ± 5.77 g, respectively. The specific growth rate of the control and treatment groups was 1.3 ± 0.02 and 1.35 ± 0.01 but the feed conversion ratio did not show a significant difference. Hemoglobin and erythrocytes showed a significant increase compared to the control in treatments containing immune stimulants and supplementation (p < 0.05). White blood cells ranged from 41417 ± 2964.84 in the treatment to 42500 ± 683.13 in the control treatment. The number of red blood cells in the control treatment was 2810000 ± 19.1763 and the recipient treatment was 3951667 ± 14588.84. Immune system stimulants including vitamins E, C and selenium can play a role in improving the growth, blood, biochemical and safety indices of farmed Late calcarifer.

https://janb.guilan.ac.ir/article_4713_9020b99a13ed6f4e8e925e09b1b3f69f.pdf