The effect of blend of isolated bacillus and Saccharomyces cerevisiae from gut of Beluga (Huso huso) on growth and feeding parameters of silver carp (Hypophthalmichthys molitrix) larvae

Document Type : Research Paper


Faculty of Agriculture and Natural Resources, University of Gonbad Kavoos, Gonbad, Golestan, Iran


A 33-day feeding experiment was conducted to assess the effects of supplemented diets with sturgeon intestinal bacteria as probiotics in feeding of silver carp (Hypophthalmichthys molitrix) larvaewith average weight 200 ± 10 mg. These probiotics were isolated from gut of ten healthy fingerlings of Beluga (Huso huso) with average weight of (5.51 ± 0.31 g). Four diets (S1, S2, S3 and S4) were prepared containing similar ingredient composition and were supplemented with blend of three probiotics (Bacillus licheniformis, Bacillus subtilis and yeast of Saccharomyces cerevisiae) at four different levels (0, 1.5×106, 3×106, 4.5×106 cells per 100 g of diet, respectively). At the end of experiment, the Silver carp larvae fed probiotic supplemented diet had an improved and growth feeding performance compared to fish larvae not fed probiotics. Rearing of silver carp with feeding by experimental diets of, S2 and S3 (3×106 and 4.5×106 cells 100 g) resulted in better growth and feeding parameters in compared with the control. These probiotics resulted in a significant increase (P<0.05) in specific growth rate and food conversion efficiency of fish larvae in experimental treatments. Therefore the tested microorganisms in this study had tremendous potential as probiotic for commercially produced of silver carp larvae.