Comparison of continuous and pulse administration of probiotic bacteria potency of Lactobacillus sp., Bacillus thuringiensis and B. cereus in the basal diet on biochemical parameters of Lates calcarifer

Mohammadian, Takavar; Oskooie Nejad Monfared, Ghazal; Razi Jalali, Mohammad; Torfi, Mansour

doi:10.22124/janb.2023.23679.1181

Comparison of continuous and pulse administration of probiotic bacteria potency of Lactobacillus sp., Bacillus thuringiensis and B. cereus in the basal diet on biochemical parameters of Lates calcarifer

Document Type : Research Paper

Authors

¹ Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan, Iran Excellence Center of Warm Water Fish Health, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan, Iran

² Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Khuzestan, Iran

³ South Iran Aquaculture Research Centre, Iranian Fisheries Science Institute (IFSRI), Agricultural Research Education and Extension organization (AREEO), Ahwaz, Khuzestan, Iran

10.22124/janb.2023.23679.1181

Abstract

This study was conducted with the aim of determining the effect of different probiotics administration on alterations in biochemical parameters of juvenile sea bass (Lates calcarifer). Four hundred and twenty sea bass with an average weight of 34 ± 4.5 g were prepared and randomly divided into seven groups with three replications (40 fish in each replication) and fed with experimental diets for 90 days. The experimental treatments included: group 1 (T₁): different Lactobacillus plantarum sp.; group 2 (T₂): L. plantarum sp., L. bulgaricus, L. acidophilus and L. rhamnosus in a combined form (combination of lactobacilli); group 3 (T₃): the combination of diets 1 and 2 as well as Bacillus cereus and B. thuringiensis which was administered to fish every two weeks; group 4 was the control group without probiotics (C₄). In addition, groups 5, 6 and 7 (T₅, T₆ and T₇) were similar to groups 1, 2 and 3 which were continuously fed to fish. The results showed that there was a significant increase in blood serum cholesterol at the end of the experiment in T₇ compared to the control group. The calcium level in T₃ showed the highest amount compared to other treatments. All treatments showed a decreased amount of total protein at the end of the experiment compared to the control (C₄). Moreover, the highest value was related to T₆. T₇ exhibited the highest amount of triglycerides and glucose at the end of the experiment. In case of liver enzymes, the lowest serum ALP level was found in T₁ and the lowest AST in T₆, while serum ALT level did not display any significant difference in any group. In cases of Cpk and HDL, the lowest values belonged to T₁. In general, it can be concluded that the use of a mixture of probiotics in a pulse administration can cause positive effects on the blood biochemical parameters of L. calcarifer.

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