Document Type : Research Paper
Authors
1
Khorramshahr University of Marine Science and Technology
2
Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology
3
Department of Marine Biology, Faculty of Marine Science, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran
4
Department of Clinical Sciences and Excellence Center of Warm Water Fish Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
10.22124/janb.2024.26403.1230
Abstract
The purpose of the present study was to replace the red algae on the diet with enzymes and its impact on the tissue of the Nile tilapia gastrointestinal tract. For this purpose, 120 pieces of tilapia fish with an average weight of 30±1 gr after preparation for 6 weeks in 100-liter aquariums with biomare diet containing zero, 3 %, 6 %, and 9 % of food weight by three red algae each treatment was fed. After the end of the breeding period, the fish was anesthetized by cloves and after opening the abdominal area of 0.5 cm from the middle of the esophagus the primary, middle, and end of the intestine were removed and placed in 10 % formalin buffer solution. After going through the routine steps of tissue preparation, including dehydration with alcohol, clarification with xylol, blocking with paraffin, cutting with a thickness of 4-6 microns with a microtome, and hematoxylin-eosin staining, finally, the study was done with a light microscope. Histometric studies in the mid-esophagus, initial, middle, and end regions of the intestine showed that the highest amount of mucous cells, the highest thickness of the epithelium and muscle layer, as well as the highest height and thickness of the esophageal villi in the group receiving the diet containing 9% red algae. Also, protease, amylase and lipase enzymes in the treatment groups had a higher level than the control group, with the difference that the amount of protease was higher than amylase and lipase enzymes. According to the results of the present study, it can be said that the possibility of using red algae in the diet of Nile tilapia has no side effects at the mentioned level, and its use in this type of fish improved tissue and growth parameters.
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