Evaluation of antimicrobial properties of diethyl ether and methanol extracts of Scenedesmus dimorphus against bacterial Micrococcus lutesus  and Aeromonas hydrophila

Habibi, Zahra; Imanpour Namin, Javid; Remazanpour, Zohreh

doi:10.22124/janb.2017.3154

Evaluation of antimicrobial properties of diethyl ether and methanol extracts of Scenedesmus dimorphus against bacterial Micrococcus lutesus and Aeromonas hydrophila

Document Type : Research Paper

Authors

¹ Fisheries Department, Faculty of Natural Resources, University of Guilan, Sowmeh Sara, Guilan, Iran

² International Sturgeon Research Organization of the Caspian Sea, Agricultural Research, Education and Extension Organization, Rasht, Guilan, Iran

10.22124/janb.2017.3154

Abstract

Antimicrobial properties of diethyl ether and methanol extracts of algae Scenedesmus dimorphus against Gram positive bacterium Micrococcus lutesus and Gram negative becterium, Aeromonas hydrophila were evaluated. The algae was pure cultured and once the required volume and number of cells achieved coli using agar well diffusion method diethylether and hexane extracts of the algae cells were extracted. Antibacterial properties of the extracts were evaluated using well diffusion method. The results showed inhibitory activities of methanol and ether extracts of Scenedesmus dimorphus against M. luteus and A. hydrophila with 11-13 mm and 13-21 mm diameters of inhibition zone, respectively. In case of diethyl ether extracts also the inhibitory activity observed with diamaters of inhibition zone in the range of 9-23.7 mm and 21.6-6.3 mm respectively against M. luteus and A. hydrophila. Gas chromatography mass spectrophotometry was used to identify different substances within diethyl ether and methanol extracts with hydrocarbon and ester with highest perentage among other substances. The results confirmed antimicrobial activities of extracts from S. dimorphus against bacteria used in this study.

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