Effects of dietary rainbow trout visceral protein hydrolysate on hematological, immunological and biochemical serum parameters and antioxidant enzymes of juvenile Siberian sturgeon, Acipenser baerii

Yeganeh, Sakineh; Adel, Milad

doi:10.22124/janb.2022.23264.1176

Effects of dietary rainbow trout visceral protein hydrolysate on hematological, immunological and biochemical serum parameters and antioxidant enzymes of juvenile Siberian sturgeon, Acipenser baerii

Document Type : Research Paper

Authors

Sakineh Yeganeh ¹
Milad Adel ²

¹ Department of Fisheries, Faculty of Animal Science and Fisheries, Sari Agricultural Sciences and Natural Resources University, Sari, Mazandaran, Iran

² Department of Aquatic Animal Health and Diseases, Iranian Fisheries Research Organization, Agriculture Research Education and Extension Organization, Tehran, Iran

10.22124/janb.2022.23264.1176

Abstract

This study evaluated the effect of different levels of dietary rainbow trout by-product protein hydrolysate on hematological, immunological and biochemical serum parameters and antioxidant enzymes of juvenile Siberian sturgeon (Acipenser baerii). For this purpose, 300 fish with an average weight of 67 ± 3 g were distributed in 12 tanks and were fed for 56 days with different levels of by-product protein hydrolysate (PH) in 4 treatments including 0 (control), 5g (5PH), 10g (10PH) and 20g (20PH per kg of feed. The highest white blood cell count and hematocrit percentage were observed in the treatments of 10PH and 20PH which was significantly different from those fed with 5PH and control (p<0.05). The highest amount of the serum glucose and the lowest amount of triglyceride were obtained in 10PH and 20PH. Fish fed 10PH and 20PH exhibited lower levels of AST and ALP enzymes than the control group (p<0.05). Evaluation of serum immune indices showed that there was a significant difference in IgM, C₃, ACH₅₀ and serum respiratory burst activity of fish fed 10PH compared to other treatments (p<0.05). Also, lysozyme activity, total protein content, complement component C₄ in fish fed with 10PH and 20PH displayed a significant difference with 5PH and control group (p<0.05). In this study, serum superoxide dismutase and catalase indices in 10PH and 20PH revealed a significant difference with other treatments (p<0.05), in addition to the activity of the glutathione peroxidase (p<0.05). Overall, effects of PH, especially 10PH on the examined indices in Siberian Sturgeon were positive and significant.

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