Document Type : Research Paper
Authors
1
Student of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran.
2
, Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran
3
Dept. of Aquaculture, Faculty of Fisheries and Environmental Sciences, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Golestan, Iran
10.22124/janb.2024.27503.1244
Abstract
The purpose of this research was to observe the effects of using chitosan on the performance of immune indicators and antioxidant defense system in Vannamei shrimp (Litopenaeus vannamei). For this purpose, 180 pieces of vanami shrimp with an average weight of about 4 grams were prepared and kept for 2 weeks to adapt to the test conditions. This study was conducted with 4 groups, each group with three repetitions, including the base diet of zero (control), 1 gram (treatment 1), 2 grams (treatment 2) and 4 grams (treatment 3) of chitosan per kilogram of feed in a randomized design. It was done for 8 weeks. At the end of the course, serum immune and antioxidant indices were measured. Immune indices such as lysozyme and serum immunoglobulin had a significant increase compared to the control group (P<0.05); Thus, in lysozyme enzyme, the highest amount related to the treatment of 2 g/kg and in immunoglobulin enzyme, the highest amount was observed in the treatment of 1 g/kg of feed. Serum antioxidant activity such as superoxide dismutase (1 and 2 g/kg), catalase (1, 2 and 4 g/kg) increased significantly compared to the control group (P<0.05). Also, the results of the serum biochemical factors analysis only in the ALP enzyme of the treatments had a significant difference (P < 0.05) and the highest value related to the treatment of 4 grams of chitosan per kilogram of feed was observed. There was no significant difference in AST and ALT enzymes (P>0.05). The results of this research indicate the positive effects of chitosan on immune indices in Vanami shrimp. Therefore, it can be recommended to use chitosan as a natural and immune stimulant for Vanami shrimp breeding.
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