تأثیر جیره حاوی پودر و عصاره اتانولی گیاه سرخارگل (Echinacea purpurea) بر فاکتورهای ایمنی غیر اختصاصی-ضداکسایشی همولنف میگوی سفید غربی (Litopenaeus vannamei) در مواجهه با استرس pH

نوع مقاله : مقاله پژوهشی

نویسندگان

گروه شیلات، دانشکده علوم و فنون دریایی، دانشگاه هرمزگان، بندرعباس، هرمزگان

چکیده

گیاه سرخارگل (Echinacea purpurea) به دلیل دارا بودن ترکیبات فنولی، پلی ساکاریدها و آلکالوئیدها به‌عنوان یک مکمل طبیعی در بهبود عملکرد دستگاه ایمنی و مقاومت در برابر استرس ­های محیطی در آبزی پروری قابل استفاده است. در این تحقیق، تأثیر جیره ­های حاوی عصاره و پودر گیاه سرخارگل بر عملکرد دستگاه ایمنی-ضد اکسایشی همولنف میگوی سفید غربی (Litopenaeus vannamei) در مواجهه با استرس pH بررسی شد. میگوها به مدت 68 روز با جیره‌هایی شامل غلظت‌های مختلف عصاره (25/0%، 5/0%، و 1%) و پودر (25/0%، 5%، و 10%) این گیاه تغذیه شدند و سپس به مدت 24 ساعت در معرض pH اسیدی (5/4) و قلیایی (5/9) قرار گرفتند. درpH  اسیدی و قلیایی، تعداد کل هموسیت‌ها به‌جز در تیمار حاوی 25/0% عصاره، در دیگر تیمارها به‌طور معنی‌داری کم­تر از گروه شاهد بود (P<0.05). یاخته‌های گرانولار بزرگ در پاسخ به استرس pH اسیدی تفاوت معنی‌دار بین تیمارها نشان ندادند (P>0.05)، اما در پاسخ به استرس pH قلیایی، به‌جز تیمار 25/0% عصاره، در دیگر تیمارها به‌طور معنی‌داری کم­تر از گروه شاهد بودند (P>0.05). همچنین، در شرایط استرس pH اسیدی و قلیایی، یاخته‌های نیمه‌گرانولار و هیالین و فعالیت آنزیم‌های لایزوزیم، سوپراکسید دیسموتاز، گلوتاتیون پراکسیداز و کاتالاز در غلظت‌های پایین‌تر عصاره و بالاتر پودر تفاوت معنی‌داری با گروه شاهد نداشتند (P>0.05)، اما با افزایش غلظت‌ عصـاره و کاهش پـودر، فاکتورهای ایمنی- ضد اکسایشی نسبت به گروه شاهد کاهش یافتند (P>0.05). تیمار حاوی 25/0% عصاره سرخارگل بیش­ترین تأثیر مثبت را در پاسخ ایمنی میگو به استرس pH نشان داد، به طوری ­که در مواجهه با استرس pH اسیدی و قلیایی تعداد هموسیت‌ها و فعالیت آنزیم ­های ایمنی- ضد اکسایشی در این تیمار تفاوت معنی‌داری با گروه شاهـد نداشت. ایـن تـأثیر مـثبت احـتمالاً به دلـیل وجـود متـابولیت ­های مـؤثـر به­ خصوص پـلی­فنول­ ها و پلی ­ساکاریدهای عصاره گیاه سرخارگل است. 

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effect of diet containing the purple coneflower, Echinacea purpurea extract and powder on innate immune-antioxidant factors in the hemolymph of Pacific white shrimp, Litopenaeus vannamei under pH stress

نویسندگان [English]

  • Morteza Raoofi
  • Arash Akbarzadeh
  • Ahmad Noori
  • Mohamad Niroomand
  • Leila Abdoli
Department of Fisheries, Faculty of Marine Sciences and Technology, University of Hormozgan, Hormozgan, Bandar Abbas, Hormozgan, Iran
چکیده [English]

Introduction: One of the novel approaches in stress management and health improvement in aquatic organisms is the use of dietary supplements with immune-boosting properties. Purple coneflower, Echinacea purpurea, as one of the medicinal plants, due to its phenolic compounds, glycosides, alkaloids, and antioxidant, anti-inflammatory, and immune-boosting properties, can be used as a natural supplement to improve immune system performance and resistance against environmental stresses in aquaculture. Research in aquatic species has shown that the use of medicinal plant including E. purpurea can have positive effects on the physiological status, immunity, and health of aquatic organisms when faced with environmental stresses and diseases. In this study, the effects of a diet containing purple coneflower extract and powder on hematological and immune-antioxidant factors of the hemolymph of Pacific white shrimp (Litopenaeus vannamei) under pH stress were investigated.
Materials and Methods: Shrimp were fed with diets containing different concentrations of purple coneflower extract (0.25%, 0.5%, and 1%) and powder (0.25%, 5% and 10%) for 68 days. Subsequently, the performance of blood and immune-antioxidant factors in the hemolymph, including total and differential hemocyte counts and the activity of enzymes such as lysozyme, superoxide dismutase, glutathione peroxidase, and catalase were evaluated after exposure to acidic (pH 4.5) and alkaline (pH 9.5) stress conditions for 24 hours.
Results and Discussion: Under acidic and alkaline pH conditions, the total hemocyte count was significantly lower than in the control group in different treatments except for treatment containing 0.25% extract of purple coneflower (P<0.05). The large granular cells did not show significant difference among treatments in response to acidic pH stress (P>0.05), however, they were significantly lower than the control group in different treatments except for treatment containing 0.25% extract of purple coneflower in response to alkaline pH stress (P<0.05). Additionally, the results showed that under acidic and alkaline pH stress conditions, the semigranular and hyaline cells and the activity of lysozyme, superoxide dismutase, glutathione peroxidase, and catalase, did not show significant differences compared to the control group at lower doses of extract and higher doses of purple coneflower (P>0.05). However, with an increase in extract dosage and a decrease in powder dosage, the immune-antioxidant factors in the shrimp's hemolymph were reduced compared to the control group.
The results of this study indicate that purple coneflower, at certain concentrations, led to changes in the hemolymph immune-antioxidant response under acidic and alkaline pH conditions, resulting in a reduction in hemocyte counts and then activity of immune-antioxidant enzymes. The results showed that the plant, at some doses, was not effective in regulating the immune-antioxidant response of shrimp under acidic and alkaline pH conditions.
Conclusion: The results of this study indicated that different concentrations of purple coneflower extract and powder can affect the immune-antioxidant system of shrimp in response to pH stress. The treatment with 0.25% the purple coneflower extract exhibited the most positive effect on shrimp immunity in response to pH stress, where hemocyte counts and immune-antioxidant enzyme activity in this treatment did not differ significantly from the control group under both acidic and alkaline pH stress. This positive effect is likely due to the presence of active metabolites, especially active polyphenolic compounds and polysaccharides, in the purple coneflower extract. When using the purple coneflower as a natural supplement in shrimp feeding, their concentrations should be carefully managed to prevent negative impacts on the immune system.

کلیدواژه‌ها [English]

  • Antioxidants
  • pH stress
  • Echinacea purpurea
  • Hemolymph
  • Shrimp

مراجع

Abdel Rahman, A.N., Khalil, A.A., Abdallah, H.M., El-Hady, M. 2018. The effects of the dietary supplementation of Echinacea purpurea extract and/or vitamin C on the intestinal histomorphology, phagocytic activity, and gene expression of the Nile tilapia. Fish & Shellfish Immunology 82: 312-318. DOI: 10.1016/j.fsi.2018.08.024.
 
Akbary, P., Kakoolaki, S. 2019. Growth, hematological, innate immune responses of Mugil cephalus fed with Echinacea purpurea-supplemented diet against Photobacterium damselae infections. International Journal of Environmental Science and Technology 16: 325-334. DOI: 10.1007/s13762-017-1578-6
 
Al Eissa, A., Chen, P., Brown, P.B., Huang, J.Y. 2022. Effects of feed formula and farming system on the environmental performance of shrimp production chain from a life cycle perspective. Journal of Industrial Ecology 26: 2006-2019. DOI: 10.1111/jiec.13370.
 
Al Mutawa, M.Y., Ayaad, T.H., Shaurub, E.H. 2020. Hemocyte profile, phagocytosis, and antibacterial activity in response to immune challenge of the date fruit stalk borer, Oryctes elegans. Invertebrate Survival Journal 17: 147-162. DOI: 10.25431/1824-307X/isj. v0i0.147-162.
 
Alinezhad, S. 2019. Effects of diets containing dry extracts of Achillea millefolium, Mentha piperita and Echinacea purpurea on growth, hematological and immunological indices in juvenile common carp (Cyprinus carpio). Iranian Journal of Aquatic Animal Health 5: 1-16. DOI: 10.29252/ijaah.5.1.1.
 
Baniesmaeili, Y., Akbarzadeh, A., Abdollahi, F., Niroomand M. 2023. Effectiveness of dietary Moringa oleifera leaf powder and extract in the Pacific white shrimp (Litopenaeus vannamei). Iranian Journal of Fisheries Sciences 22: 547-565. DOI: 10.22092/ijfs.2023.129278.
 
Cargnin, J.M.R., João, J.J. 2024. Biological denitrification and physico-chemical treatment for removing contaminants and toxicity in wastewater generated by shrimp farming. International Journal of Environmental Science and Technology 21: 6287-6296. DOI: 10.1007/s13762-023-05424-4.
 
Ciji, A., Akhtar, M.S. 2021. Stress management in aquaculture: A review of dietary interventions. Reviews in Aquaculture 13: 2190-2247. DOI: 10.1111/raq.12565.
 
Delphino, M.K., Laurin, E., Patanasatienkul, T., Rahardjo, R.B., Hakim, L., Zulfikar, W.G., Burnley, H., Hammell, K.L., Thakur, K. 2022. Description of biosecurity practices on shrimp farms in Java, Lampung, and Banyuwangi, Indonesia. Aquaculture 556: 738277. DOI:10.1016/j.aquaculture.2022.738277.
 
Fierro-Coronado, J.A., Luna-González, A., Cáceres-Martínez, C.J., Ruiz-Verdugo, C.A., Escamilla-Montes, R., Diarte-Plata, G., Flores-Miranda, M.D.C., Álvarez-Ruiz, P. Peraza-Gómez, V. 2019. Effect of medicinal plants on the survival of white shrimp (Penaeus vannamei) challenged with WSSV and Vibrio parahaemolyticus. Latin American Journal of Aquatic Research 47: 377-381. DOI: 10.3856/vol47-issue2-fulltext-20.
 
Fox, J.M., Lawrence, A.L., Smith, F. 2004. Development of a low-fish meal feed formulation for commercial production of Litopenaeus vannamei. Avances En Nutrición Acuicola. https://nutrici onacuicola.uanl.mx/index.php/acu/article/view/199
 
Fox, J.M., Treece, G.D., Sanchez, D.R. 2001. Shrimp nutrition and feed management. Methods for improving shrimp farming in Central America. University of Central America Press, Managua, Nicaragua, 65-90.
 
Geetha, R., Avunje, S., Solanki, H.G., Priyadharshini, R., Vinoth, S., Anand, P.R., Ravisankar, T., Patil, P.K. 2022. Farm-level economic cost of Enterocytozoon hepatopenaei (EHP) to Indian Penaeus vannamei shrimp farming. Aquaculture 548: 737685. DOI:  10.1016/j.aquaculture.2021.737685.
 
Gong, H., Qin, Z., Chen, Z., Li, J., Chang, Z., Li, J., Chen, P. 2022. Effects of dietary tannic acid on growth, digestion, immunity and resistance to ammonia stress, and intestinal microbial community in Pacific white shrimp (Litopenaeus vannamei). Fishes 7: 327. DOI: 10.3390/fishes7060327.
 
Hong, Y., Yin, H., Huang, Y., Huang, Q., Yang, X. 2020. Immune response to abamectin-induced oxidative stress in Chinese mitten crab, Eriocheir sinensis. Ecotoxicology and Environmental Safety 188: 109889. DOI: 10.1016/j.ecoenv.2019.109889.
 
Hou, R., Xu, T., Li, Q., Yang, F., Wang, C., Huang, T., Hao, Z. 2020. Polysaccharide from Echinacea purpurea reduce the oxidant stress in vitro and in vivo. International Journal of Biological Macromolecules 149: 41-50. DOI:  10.1016/j.ijbiomac.2020.01.129.
 
Kathyayani, S.A., Poornima, M., Sukumaran, S., Nagavel, A., Muralidhar, M. 2019. Effect of ammonia stress on immune variables of Pacific white shrimp Penaeus vannamei under varying levels of pH and susceptibility to white spot syndrome virus. Ecotoxicology and Environmental Safety 184: 109626. DOI: 10.1016/j.ecoenv.2019.109626.
 
Medina-Beltrán, V., Luna-González, A., Fierro-Coronado, J.A., Campa-Córdova, Á.I., Peraza-Gómez, V., del Carmen Flores-Miranda, M., Rivera, J.N.G. 2012. Echinacea purpurea and Uncaria tomentosa reduce the prevalence of WSSV in whiteleg shrimp (Litopenaeus vannamei) cultured under laboratory conditions. Aquaculture 358: 164-169. DOI: 10.1016/j.aquaculture.2012.06.030
 
Miles, R.D., Chapman, F.A. 2006. The benefits of fish meal in aquaculture diets. DOI: 10.32473/edis-fa122-2006
 
Miroshina, T., Poznyakovskiy, V. 2023. Echinacea purpurea as a medicinal plant: characteristics, use as a biologically active component of feed additives and specialized foods. In E3S Web of Conferences 380: 01005. EDP Sciences. DOI: 10.1051/e3sconf/202338001005.
 
Moghadam, H., Sourinejad, I., Johari, S.A. 2022. Dietary turmeric, curcumin and nanoencapsulated curcumin can differently fight against salinity stress in Pacific white shrimp Penaeus vannamei Boone, 1931. Aquaculture Research 53: 3127-3139. DOI: 10.1111/are.15825.
 
Niroomand, A., Akbarzadeh, A., Ebrahimi, S.A., Sobhani, Sheikhahmadi, A. 2020. Effects of dietary black cumin seed meal on growth performance, blood biochemistry and fatty acid composition of Pacific white shrimp Litopenaeus vannamei. Aquaculture Nutrition 26: 1072-1082. DOI: 10.1111/anu.13065.
 
Oniszczuk, T., Oniszczuk, A, Gondek, E., Guz, L., Puk, K., Kocira, A., Kusz, A., Kasprzak, K., Wójtowicz, A.  2019. Active polyphenolic compounds, nutrient contents and antioxidant capacity of extruded fish feed containing purple coneflower Echinacea purpurea (L.) Moench. Saudi Journal of Biological Sciences 26: 24-30. DOI: 10.1016/j.sjbs.2016.11.013.
 
Oskoii, S.B., Kohyani, A.T., Parseh, A., Salati, A.P., Sadeghi, E. 2012. Effects of dietary administration of Echinacea purpurea on growth indices and biochemical and hematological indices in rainbow trout (Oncorhynchus mykiss) fingerlings. Fish Physiology Biochemical 38: 1029-1034. DOI: 10.1007/s10695-011-9587-8
 
Petrova, A., Ognyanov, M., Petkova, N., Denev, P. 2023. Phytochemical characterization of purple coneflower roots, Echinacea purpurea (L.) Moench. and their extracts. Molecules 28: 3956. DOI: 10.3390/molecules28093956.
 
Qiu, J., Wang, W.N., Wang, L.J., Liu, Y.F., Wang, A.L. 2011. Oxidative stress, DNA damage and osmolality in the Pacific white shrimp, Litopenaeus vannamei exposed to acute low temperature stress. Comparative Biochemistry and Physiology 154C: 36-41. DOI: 10.1016/j.cbpc.2011.02.007.
 
Rahman, R.A., Kendall, G., Ramli, R., Jamari, Z., Ku-Mahamud, K.R. 2017. Shrimp feed formulation via evolutionary algorithm with power heuristics for handling constraints. Complexity 2017: 7053710. DOI: 10.1155/2017/7053710.
 
Tang, X.L., Fu, J.H., Li, Z.H., Fang, W.P., Yang, J.Y., Zou, J.X. 2016. Effects of a dietary administration of purple coneflower (Echinacea purpurea) on growth, antioxidant activities and 8 miRNAs expressions in crucian carp (Carassius auratus). Aquaculture Research 47: 1631-1638. DOI: 10.1111/are.12624
 
Thomsen, M.O., Fretté, X., Christensen, L.P., Grevsen, K. 2010. The impact of cultivation techniques on bioactive compounds in the aerial parts of Echinacea purpurea. 28th International Horticultural Congress (IHC). Lisboa, Portugal, p 70.
 
Wang, Z., Qu, Y., Yan, M., Li, J., Zou, J., Fan, L. 2019. Physiological responses of Pacific white shrimp Litopenaeus vannamei to temperature fluctuation in low-salinity water. Frontiers in Physiology 10: 1025. DOI: 10.3389/ fphys.2019.01025
 
Wang, Z., Zhou, J., Li, J., Zou, J., Fan, L. 2020. The immune defense response of Pacific white shrimp (Litopenaeus vannamei) to temperature fluctuation. Fish & Shellfish Immunology 103: 103-110. DOI: 10.1016/j.fsi.2020.04.053.
 
Yang, Y., Xu, W., Jiang, Q., Ye, Y., Tian, J., Huang, Y., Du, X., Li, Y., Zhao, Y., Liu, Z. 2022. Effects of low temperature on antioxidant and heat shock protein expression profiles and transcriptomic responses in crayfish (Cherax destructor). Antioxidants 11: 1779. DOI: 10.3390/antiox11091779
 
Yu, Q., Xie, J., Huang, M., Chen, C., Qian, D., Qin, J.G., Chen, L., Jia, Y., Li, E. 2020. Growth and health responses to a long-term pH stress in Pacific white shrimp Litopenaeus vannamei. Aquaculture Reports 16: 100280. DOI: 10.1016/j.aqrep.2020.100280
 
Zhu, Y., Lao, F., Pan, X., Wu, J. 2022. Food protein-derived antioxidant peptides: Molecular mechanism, stability and bioavailability. Biomolecules 12: 1622. DOI: 10.3390/biom12111622.

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