نوع مقاله : مقاله پژوهشی
نویسندگان
انستیتو تحقیقات بین المللی ماهیان خاویاری، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی (AREEO)، رشت، گیلان،
چکیده
کلیدواژهها
موضوعات
عنوان مقاله [English]
نویسندگان [English]
This study aimed to investigate the effect of different levels of selenium nanoparticles and iron nanoparticles levels on the histopathology of the intestine of the beluga juvenile. 135 beluga juveniles (with initial weight of 276.4±32.3 g and initial length of 40.0±2.0 cm) were randomly distributed in 27 fiberglass tanks at 9 different treatments with 3 replicates. Treatments including T1 (control, no added NPs), T2 (1 mg/kg Se-NPs), T3 (2 mg/kg Se-NPs), T4 (100 mg/kg Fe-NPs); T5 (100 mg/kg Fe-NPs +1 mg/kg Se-NPs); T6 (100 mg/kg Fe-NPs +2 mg/kg Se-NPs); T7 (200 mg/kg Fe-NPs); T8 (200 mg/kg Fe-NPs +1 mg/kg Se-NPs); T9 (200 mg/kg Fe-NPs +2 mg/kg Se-NPs). Before starting the experiment, the fish were adapted to the rearing conditions for a week. The amount of feeding during the rearing period was 2-3% based on the weight and water temperature, and the fish were fed three times a day (8:00-15:00 and 21:00) for 8 weeks. In order to find the histopathological changes, six fish intestine tissues from each group (two fish of each replicate) were sampled at the end of the experiment. Samples were dehydrated by routine methods and embedded in paraffin wax. They were sectioned by microtome and stained with H & E. The complications observed in the intestinal tissue included degeneration of villi, infiltration of inflammatory cells in the submucosa layer, infiltration of inflammatory cells in the muscle layer, hyperemia (under the serous layer) and necrosis in the muscle layer. Based on the observed results, in total, the lowest amount of damage was observed in treatment T8 (equal to the control) and then treatment T5. So it can be concluded that in the combined use of iron and selenium nanoparticles, doses of 100 mg/kg of iron nanoparticles and 1 mg/kg of selenium nanoparticles in beluga diet are more appropriate.
کلیدواژهها [English]
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