اثر سطوح مختلف ضد اکسایش رسوراترول (Resveratrol) بر فراسنجه های خونی، شاخص های بیوشیمیایی سرم و آنزیم های کبدی بچه ماهی ازون برون (Acipenser stellatus)

نوع مقاله : مقاله پژوهشی

نویسندگان

1 گروه شیلات، واحد لاهیجان، دانشگاه آزاد اسلامی، لاهیجان، ایران

2 انستیتو تحقیقات بین المللی ماهیان خاویاری، موسسه تحقیقات علوم شیلاتی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، رشت، ایران

10.22124/janb.2026.33913.1311

چکیده

ترکیبات زیست ­فعال موجود در گیاهان، به واسطه دارا بودن خواص ضداکسایشی و ضدالتهابی، نقش مهمی در تغذیه آبزیان دارند. این مطالعه با هدف بررسی اثـرات ضـد­اکسایشی رسوراترول بر فـراسنجه­های خونی، شاخص­های بیوشیمیایی سرم و آنزیم­های کبدی بچه ماهی ازون­برون (Acipenser stellatus) انجام شد. تعداد 150 ماهی با میانگین وزنی 5/2 ± 92 گرم در یک طرح کاملاً تصادفی با 5 تیمار آزمایشی و 3 تکرار (با تعداد 10 عدد در هر تکرار) استفاده شد که شامل گروه شاهد (R0؛ بدون استفاده از رسوراترول) و تیمارهای آزمایشی با تغذیه از رسوراترول به ترتیب 200، 400، 800 و mg/kg 1000 غذا (به ترتیب R200، R400، R800 و R1000) و به مدت 8 هفته تغذیه شدند. نتایج حاصله نشان داد که در پایان آزمایش، بیشترین میزان هماتوکریت 42/2±  66/42 % در تیمار حاوی mg/kg1000 رسوراترول (R1000) مشاهده شد که نسبت به گروه شاهد دارای تفاوت معنی­دار بود (05/0P<). کمترین میزان گلوکز و کلسترول در تیمارهای حاوی 800 و mg/kg 1000 رسوراترول (R800 و R1000) مشاهده شد که با گروه شاهد دارای تفاوت معنی­دار بود (05/0P<). همچنین کمترین میزان تری­گلیسرید در تیمار حاوی  mg/kg1000 رسوراترول مشاهده شد که با گروه شاهد دارای تفاوت معنی­دار بود (05/0P<). کمترین مقدار آنزیم کبدی (AST) aspartate aminotransferase در تیمار حاوی mg/kg 1000 رسوراترول مشاهده شد که با گروه شاهد دارای تفاوت معنی­دار بود (05/0P<). در مجموع، بر اساس نتایج این تحقیق، افزودن  mg/kg1000 رسوراترول به جیره غذایی بچه ماهی ازون­برون برای بهبود فراسنجه­های خونی، شاخص­های بیوشیمیایی سرم و آنزیم های کبدی در این ماهی پیشنهاد می­ شود.

کلیدواژه‌ها

موضوعات

عنوان مقاله [English]

Effects of different levels of resveratrol antioxidant on blood parameters, serum biochemical indices and liver enzymes of juvenile Stellate sturgeon, Acipenser stellatus (Pallas, 1771)

نویسندگان [English]

  • Younes Golalipour 1
  • Hossein Khara 1
  • Habib Vahabzadeh Roudsary 1
  • Ayoub Yousefi Jourdehi 2
  • Alinaghi Sarpanah 2
1 Department of Fisheries, La.C., Islamic Azad University, Lahijan, Iran
2 International Sturgeon Research Institute, Agricultural Research Education and Extension Organization (AREEO), Rasht, Iran
چکیده [English]

Introduction: Bioactive compounds in plants are essential for aquatic animal nutrition because of their antioxidant and anti-inflammatory effects. They combat oxidative stress by neutralizing free radicals and protecting cells from damage. This protection helps to maintain normal physiological functions critical for fish health. Furthermore, these compounds enhance the immune system, increasing fish resistance to pathogens and environmental stressors. Improved immunity reduces disease risk and mortality rates. Bioactive substances also support digestion and nutrient absorption, as well as promoting better growth performance. Collectively, they boost fish resilience and biological functioning, making them valuable for sustainable aquaculture. Given the importance of Stellate sturgeon, Acipenser stellatus, as one of the most commercially valuable fish species in the Caspian Sea, in this research, the effects of different levels of resveratrol antioxidant on blood parameters, serum biochemical indices, and liver enzymes in the juvenile Stellate sturgeon were studied for 8 weeks.
Materials and Methods: To examine the effects of Resveratrol, five dietary treatments with 200, 400, 800, and 1000 mg/kg (R200, R400, R800, and R1000, respectively) were prepared along with a control basal diet without Resveratrol (R0). Ingredients for the basal diet were precisely weighed and mixed, with the Resveratrol added accordingly. Pellets were made by mixing the ingredients, forming dough with water, grinding through a 2 mm pellet, drying at 40 °C for 36 h, and storing at dry and cold place. One hundred and fifty Stellate sturgeon averaging 92 ± 2.5 g were acclimated for two weeks in 15 fiberglass tanks stocked with 10 fish each. After acclimation, tanks were randomly assigned to treatments with three replicates for an 8-week trial. Water quality was maintained with aeration, daily 50% water exchange, and stable temperature (18.54 ± 0.53 °C), oxygen (6.89 ± 0.19 mg/L), and pH (8.09 ± 0.01). At the end, in order to determine the hematological, biochemical parameters, and liver enzymes, blood was taken from the caudal vein using a syringe and poured into a 2-mL microtube. Data were statistically analyzed using One-Way ANOVA, and Tukey’s multiple comparison with significance at P<0.05.
Results: The results showed that at the end of the experiment, the highest hematocrit (42.66 ± 2.42%) was observed in R1000, exhibiting a significant difference compared to the control (R0; P<0.05). The lowest glucose and cholesterol levels were observed in R800 and R1000, exhibiting a significant difference compared to the control (R0; P<0.05). Also, the lowest triglyceride was observed in R1000, exhibiting a significant difference compared to the control (R0; P<0.05). The lowest aspartate aminotransferase (AST) was observed in R1000, exhibiting a significant difference compared to the control (R0; P<0.05).
Discussion and Conclusion: The results showed that Resveratrol can improve the general health status of aquatic animals, but it is very important to determine the effective doses on blood indices, serum biochemical parameters, and liver enzymes. Therefore, in the present study, Resveratrol in nutritional treatments with a dose of 1000 mg/kg diet is recommended for juvenile Stellate sturgeon. Overall, the present results suggest that a diet containing 1000 mg/kg Resveratrol could enhance blood indices, serum biochemical parameters, and liver enzymes in juvenile Stellate sturgeon.

کلیدواژه‌ها [English]

  • Resveratrol
  • Blood indices
  • Liver enzymes
  • Biochemical parameters
  • Acipenser stellatus

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